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Further experimental studies using other techniques (e. g. pulse- chase immunoprecipitation and GFP- based methods) are needed, not only to confirm protein import into Paulinella photosynthetic entities, but also to clarify how it occurs.
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A likelihood based method is needed.
Therefore, sequence-based methods are needed.
For nonlinear models with no closed-form expression for dose, simulation-based methods are needed.
ASTRAL provides one such advance, but new and more accurate coalescent-based methods are needed to enable these analyses, especially for genome-scale datasets where missing data and extremely low phylogenetic signal in individual genes may be a substantial problem.
As an alternative, fire engineering performance based methods are developed, which may need sophisticated numerical models to adequately simulate the responses of structures in the design fire scenarios.
For timely results, rapid culture and PCR based drug resistance testing methods are needed as drug resistance is acquired early during treatment.
Thus, it is obvious that methods are needed that can adapt molecular taxonomy to reference data based on traditional taxonomy, without requiring that the latter is 100% correct.
Additionally, affinity methods based on capture of exosomes using peptides or antibodies are being used as specific methods.> The current technologies are thus limited, and moving forward, simpler methods are needed.
Other methods are needed.
Therefore, alternative strengthening methods are needed.
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