Sentence examples for based method to isolate from inspiring English sources
Here, we present a microdroplet based method to isolate and pattern single cells from heterogeneous cell suspensions (10% target cell mixture), preserve viability of the extracted cells (97.0±0.8%), and obtain genomic information from isolated cells compared to the non-patterned controls.
Serum-free medium of T cells was collected and processed using a SPEG-based method to isolate formerly N-glycosylated peptides as described in the Methods section.
For example, Ghawana et al. (2011) propose a phenol-based method to isolate RNA in species with high presence of secondary metabolites such as rheum (Rheum austral D. Don) and arnebia (Arnebia euchroma (Royle) I. M. Johnst).
They used an electroporation based method to introduce plasmid DNA/double-stranded RNAs into freshly isolated Z. elegans mesophyll cells.
More recently, we have begun to take advantage of next generation Illumina DNA sequencing based methods to greatly accelerate the pace at which we can identify the affected genes in mutants isolated after mutagenesis of nematode populations.
Our lab has also established a method to isolate these TICs based on the property of increased invasive ability [ 22].
FACS is the most efficient and economical method to isolate hundreds of thousands of individual cells per minute based on their size, granularity and fluorescence properties [4] (Fig. 1c).
Based on recent evidence that NKT cells reside in liver vascular compartment, we developed a simple method to isolate NKT cells by perfusion with PBS-containing 10 mM of EDTA.
In the next step, we sought to perform a similar investigation based on two alternative methods to isolate CSC.
Therefore, the aim of this study was to determine the occurrence of Q fever abortion using a PCR based method on DNA isolated from aborted foetal abomasal contents and placental tissues from ruminants in northern Cyprus.
