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A likelihood based method is needed.
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Further experimental studies using other techniques (e. g. pulse- chase immunoprecipitation and GFP- based methods) are needed, not only to confirm protein import into Paulinella photosynthetic entities, but also to clarify how it occurs.
Although drug interaction information has been increasingly accumulated, a novel approach like network-based method is needed to analyse that information systematically and intuitively Beyond focussing on drug-drug interactions, we examined interactions between functional drug groups.
Therefore, sequence-based methods are needed.
Some other method is needed.
A better method is needed.
The results from the last experiment using even fewer participants and a new computer based method were less robust and needs to be investigated further.
One downside of HMM based methods is the need for a training set (generally originating from annotated regions of the genome) which necessarily guides the method towards the recognition of regions which are characteristically similar to the training set.
A method was needed that could identify items for procurement based on informal judgment without the need to carry out a formal and time-consuming health economic evaluation.
As an alternative, fire engineering performance based methods are developed, which may need sophisticated numerical models to adequately simulate the responses of structures in the design fire scenarios.
Nevertheless, their conclusions based on DNA-barcoding and tree based methods were limited because these methods need reliably identified specimens as reference.
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