The use of different non-array based methods for quantification of gene expression, such as reverse transcription polymerase chain reaction (RT-PCR) is also desirable.
In this work, a harmonic analysis based method for linearity assessment and noise quantification in EIS measurements is presented, and validated both from an experimental point of view and from a theoretical point of view, for Tafelian systems.
So far the antibody based methods for hepcidin quantification in human serum are either semi-quantitative [15], [16] or measure the prohormone whose correlation to the levels of hepcidin25 remains controversial [17], [18].
The development of a non-invasive, imaging-based method for quantification of the beta cell mass could greatly enhance our understanding of the complex pathophysiology underlying the development of diabetes.
The LC-ESI-MS/MS-based method for quantification of angiotensin II levels in human plasma was successfully evaluated within the study.
The SDS-PAGE-based method for quantification of protein is a method of choice due to its simplicity and reliability, whereby proteins are resolved and migrated according to their molecular weights [ 12].
The acylcarnitine platform is an UPLC-MS/MS based method that allows for the separation and quantification of several important isomers of acylcarnitine species as well as trimethylamine-N-oxide, choline, betaine, deoxycarnitine, and carnitine.
In vitro test have shown potential for the use of RP-HPLC and NMR based methods, but only for the quantification of pure fractions of cyclotides, and showed limitations for spectrophotometric methods for these peptides [ 43].
Based on this, an electrochemical method for quantification of cypermethrin has been proposed that can detect cypermethrin down to 2 ppb.The work has opened up new possibilities for electrochemical study of detoxification processes carried out by GSH.
We describe a PCR method for quantification of microRNAs based on a single reverse transcription reaction for all microRNAs combined with real-time PCR with two, microRNA-specific DNA primers.
An external method for quantification of organochlorine compounds based on retention times was used.
