Taking the average of the HEFD values calculated for all of the experimental data as the parameter for failure (EIntrinsic), creep-fatigue life predictions were made using a simplistic hysteresis energy based method as well as the time fraction/cycle fraction method endorsed by ASME Code and compared to experimental results.
Hardware implementation results show an area reduction and an improvement in the sinusoid frequency resolution of the SOS based method (as precise as 1.9 μHz in this work), when compared with previous approaches.
In addition the use of intein based methods as well as the preparation of the solid support for Staudinger ligation often require reagents such as phosphines or thiophenols that are toxic and difficult to handle.
As mentioned in the text, we also varied the threshold to the 90th or 99th percentile, and also tested the Poisson based methods as the one also used by Ernst et al. [ 19], with a significance threshold of P < 0.05 or P < 10-5.
Transient transfection of S2 cells was performed using a calcium phosphate based method as described in the Drosophila Expression System Kits manual (Invitrogen).
In order to estimate the binding interactions of Fmoc-group in the dipeptides with protein clusters, the fluorescence based method as described by Bourassa et al. was applied (under low Fmoc-YL concentration of 0.06 mM, far below that required for peptide self-assembly).
The phylogeny was constructed using a maximum likelihood based method as implemented in PhyML [ 72] using the JTT substitution model [ 73] with the default proportion of invariable sites (0.0) and rate heterogeneity between sites corrected by a gamma law (using the default gamma parameter of 1.0 and eight rate categories).
e) Frequency Domain Analysis based methods As mentioned earlier, the baseline is defined as a component of the raw Raman signal having broad bands.
It is known that, under the assumption that data is randomly missed, a likelihood based method (such as the mixed effect model) provides unbiased inference regardless of missing data.
The effect of the random variations on system response is evaluated using a non-intrusive polynomial chaos (PC) based method known as the probabilistic collocation method (PCM).
