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The infection was quantified by a CFU (colony forming unit) based method as previously described [16], [23].
The levels of the NIS mRNA were assessed by a semi-quantitative RT-PCR based method, as previously described [ 12].
Genomic DNA was extracted from myoblasts and telomere length was measured by a qPCR based method as previously described by Mamchaoui et al. (Skeletal muscle 2011).
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However, MI based methods as previously formulated, provide limited insight into the dynamic behavior of the system, and hence have limited use in predicting new observations a key property for estimating a model's relevance when the ground truth is unknown.
Gene prediction was done using a combination of ab initio and EST-alignment based methods as previously detailed [ 8, 25].
The high percentage reported by the latter group could be related to the study of a different population (lesbian women), to the use of culture methods better suited for L. crispatus and corresponds better with results obtained by non culture based methods, as reported previously [ 16, 22, 26, 27, 32, 36- 38].
Stoichiometry counting was performed using the brightness analysis method as previously described.
DNA was purified from the supernatant using a silica-based method as previously described [24] [25].
Genotyping of the mice from tail biopsies was performed using a PCR-based method, as previously described [9].
The total number of BrdU, Dcx and NeuN cells in the DG were calculated by using the Abercrombie-based method as previously reported [43], [44].
P<0.05 was considered as statistically siGenotypes difference.
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