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The Nrf2 mutants were generated by site-directed mutagenesis using the PCR and Dpn1 based method as described before [28].
However, there are some limitations with an InSAR based method as described here.
ALP and TRAP activity were determined by nitrophenol based method as described by Bessy et al. [ 15] and Godkar [ 16], respectively.
Transient transfection of S2 cells was performed using a calcium phosphate based method as described in the Drosophila Expression System Kits manual (Invitrogen).
ALP and TRAP activity were determined by nitrophenol based method as described by Bessy et al. [ 22] and Godkar [ 23] respectively.
In order to estimate the binding interactions of Fmoc-group in the dipeptides with protein clusters, the fluorescence based method as described by Bourassa et al. was applied (under low Fmoc-YL concentration of 0.06 mM, far below that required for peptide self-assembly).
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The infection was quantified by a CFU (colony forming unit) based method as previously described [16], [23].
The levels of the NIS mRNA were assessed by a semi-quantitative RT-PCR based method, as previously described [ 12].
Genomic DNA was extracted from myoblasts and telomere length was measured by a qPCR based method as previously described by Mamchaoui et al. (Skeletal muscle 2011).
To avid the problem, we used the interaction confidence score (i.e. edge weight) in the network-based method as described above.
miR-11 was re-introduced into the targeted locus using the RMCE-based method as described.
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