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A total of 640 SNP based markers were developed between Chiifu and Tetra lines.
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PCR and fragment analysis methods were as described by Nelson et al. In addition, intron polymorphism (IP) primer sequences and methods were reported by Panjabi et al. New gene-based markers were developed based on publicly available cDNA sequences for fatty acid desaturase and FLC genes.
To fine-map GLR1, new PCR-based markers were developed and 1,447 F2 glabrous plants were analyzed using markers as given in Table 1.
DNA polymorphisms discriminating yield-positive alleles and non-target alleles for each gene were selected through sequence analysis and the allele-specific PCR-gel-based markers were developed.
In total, 1 826 SNP-based markers were developed.
Polymerase chain reaction (PCR -based markers were develoPCR -basedistinguish S. tuberosumarkers
For regions associated with MRDD resistance, more PCR-based markers were developed to map target QTLs.
This generation of duplications is due to the RJPrimer tool, using which the TE-based markers were developed.
The SNP-based markers were developed from BAC-end sequence resources, and their inclusion significantly increased the density of the map.
Linkage maps containing microsatellite, AFLP and EST-based markers were developed for a table grape segregating F1 progeny and used to perform quantitative analysis in combination with phenotypic data collected over three years.
Seven sequence-specific, simple PCR-based markers were developed which flank the R gene PhtjR [ 44]; unfortunately, none have both the key characters of co-segregating and diagnostic desired for MAS.
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