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This scenario is exacerbated in the case of gene based markers such as ESTs [ 3], which are valuable for interspecies comparative studies.
28 More recently there has been a focus on developing cerebrospinal fluid based markers, such as β amyloid and tau, for changes in Alzheimer's disease that can predate the onset of the dementia, the so called prodromal phase of Alzheimer's disease.
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Molecular marker technology has advanced from laborious and expensive restriction fragment polymorphisms to high-throughput sequence bases markers such as simple sequence repeats and single nucleotide polymorphisms (SNPs) [ 3].
Expressed sequence tags (ESTs) are an important source of gene-based markers such as those based on insertion-deletions (Indels) or single-nucleotide polymorphisms (SNPs).
The development of an updated high-density integrated B. rapa genetic linkage map based on highly cross-species transferable gene-based markers such as EST-SSRs and IP markers would allow comparative genetic analysis between B. rapa and other Brassica crops.
In addition, the lab is also researching biomarkers that predict treatment response to various therapeutic interventions by examining cargoes of extracellular vesicles in correlation with brain-based markers such as those measured through EEG or MRI.
From these databases, we selected 208 PCR-based markers such as STS-RFLP and SSR markers.
In contrast to gel-based markers, hybridization-based markers such as SFP, DArT or SNP generate quantitative allele-frequency estimates.
Heyland also eluded to the use of serum-based markers, such as procalcotonin, IL-6 and IL-8, to help diagnose VAP in the future [ 33].
Therefore, the use of blood-based markers, such as circulating tumor cells (CTCs), represents a promising complementary strategy for disease monitoring.
This facilitates mapping of gene-based markers, such as ESTs, which are commonly low in polymorphism [ 12] and are valuable tools for interspecies comparative studies.
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