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A total of 33,458 gene/transcripts from the two F. x ananassa pools were predicted based in the reference model and 19,833 and 19,720 were expressed in the ripe fruits of the high γ-decalactone and the no γ-decalactone pools, respectively.
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For external quality control of urinary iodine (UI) assays 30% of urinary samples were also tested for UI content in the reference laboratory based in the Endocrinology Research Center (Moscow, Russia).
These changes give an error rate of only 1 in 50,000 bases in the reference sequence.
We then mapped these reads to the reference genome using Pash 3.0 [22] and created a comprehensive methylation map, where each cytosine base in the reference genome is annotated with the number of times methylation is observed at that position.
In addition, any putative SNPs in low-coverage sites where the majority base differed from the reference genome but the majority consisted of only 1 or 2 bases were rejected due to lack of sufficient data, and replaced with the base in the reference genome.
First, we enumerated all exact sequence repeats of ≥36 bases in the reference genome using MUMmer (version 3.23) [ 35].
To be precise about terminology, we term any base that differs from the consensus base (typically the base in the reference genome) a single nucleotide difference (SND).
In general, if a base in the reference sequence has a high enough coverage with a consistent difference from its reference, this base likely represents a polymorphism.
For each alignment, the identity level was computed as the fraction of bases in the reference sequence that are paired with identical bases in the target sequence.
Each base in the reference genomes was further classified as coding or non-coding based on Ensembl genome annotation [ 31] (drosophila melanogaster_core_37_4e, and homo sapiens core_45_36g).
The overlapping factor is calculated by the software from the two different bases in the reference and sample traces on either side of the mutation.
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