One of those techniques is the high resolution melting (HRM) curve analysis, a polymerase chain reaction (PCR) based high-throughput assay for detecting DNA sequence variation by measuring changes in the melting of a DNA duplex, that has been used successfully with DNA extracted from both frozen and paraffin-embedded tissue [10], [11], [12].
Previously, we showed that GF could induce apoptosis using our caspase-sensor based high-throughput assay in HeLa cells [ 24].
Our results demonstrated that this IFN-γ based high-throughput assay for screening of natural products for NK cell tumoricidal activity is a simple, economic and reliable method.
The same group also developed a fluorescent based high-throughput assay format that utilized a hydrophobic interaction between the P-loop and activation loop to identify both the active and inactive conformation kinase inhibitors.
Therefore, in this study, we also developed an IFN-γ based high-throughput assay screen for assessing the effect of small-molecule natural products on the tumoricidal activity of NK cells.
We designed an ELISA-based high throughput assay to rapidly screen compound libraries, and hits were confirmed in several orthogonal biochemical and cell-based assays.
The time sensitivity poses a technical problem, rendering it difficult to develop a bromocresol-based high throughput assay to measure albumin in parallel.
The antioxidant capacities of the herbal extracts were also measured using a S. cerevisiae-based high throughput assay [ 19]. S. cerevisiae BY4743 were cultured overnight in a 50 mL volume by inoculation of a single colony.
In this study, we developed a simple and economic, IFN-γ based high-throughput screening assay to identify natural products that could enhance the NK cells tumoricidal activity.
