Subsequent differentiation of these mixed cultures would fail to generate single pure lineages needed for transplantation, modeling or population based gene expression studies (e.g. RNA-seq).
It is widely believed that the abundant globin transcripts in whole blood are the causative factors for the lowered sensitivity and increased variability in microarray based gene expression studies.
Most of the microarray based gene expression studies focused on identification of differentially regulated 'genes', but not their 'alternatively spliced transcript isoforms'.
Moreover, a microarray-based gene expression study of demyelinated hippocampi revealed that there was a significant decrease in genes that are involved in axonal transport [ 108].
Five benchmark microarray based gene expression databases are used in this study: GLI-85 (also known as GSE4412 [48], GLA-BRA-180 (also known as GDS1962 [49], CLL-SUB-111 (also known as GSE2466 [50], TOX-171 (also known as GDS2261 [51], and SMK-CAN-187 (also known as GSE4115 [52].
Microarray based gene expression dataset considered for the present study consists of a total of 39 samples of the same WT condition, with 3923 genes in each sample [ 37].
Advances in molecular profiling studies using DNA-microarray based gene-expression profiling have provided increased awareness about the regulatory networks altered in human HCC and have also provided useful gene expression-based signatures that can distinguish tumor subtypes, assist clinical staging and predict patient outcomes.
Although the expression and functional activity of these metabolic enzymes has not been quantified relative to other organs, their presence at the BBB endothelium has been postulated based on gene expression studies.
Furthermore, based on gene expression studies of lupus patients, we have reported that the BM may be more informative than peripheral blood (PB) in differentiating active from inactive lupus patients and in differentiating patients from control individuals [ 9].
Based on gene expression studies of Mecp2 knockout mice and biochemical evidence, it has been suggested that the essential function of Mecp2 in the brain might not be transcriptional [ 49].
