We developed a permutation - based extension to the iHS statistic proposed by [ 9] for which there is no need to determine the ancestral and derived state of the alleles but contrasts minor and major allele.
Because of the extremely large genome size of spruce, a standard AFLP protocol, based on EcoRI- MseI digestion, EcoRI, and MseI primer extension by 1 nucleotide in the preamplification step and 3 base extension to the EcoRI and MseI primers in the selective amplification step [ 39], produced complex AFLP fragment patterns.
This analysis is summarized in Additional file 3. Briefly, we calculated the probability that the frequency with which the two most frequent (and overlapping) 6mers, TCGGCA and GGCAGT, and to the most frequent 7mer, GGCAGTT (a one base extension to the second 6mer), occur at least once or twice in the upstream regions of effector genes is by chance association.
The neuroanatomical rat brain viewer (NeuARt) is based on neuroanatomical extensions to the neurocore database system that allow neuroscientists to browse, compare, and query complex, spatially distributed patterns of the label obtained from different experiments at their desktops through a network connection.
20 In our present study, we microscopically subclassified the extent of minimal ETE to estimate and compare the effect on RFS based on extension to the perithyroid soft tissue or the sternothyroid muscle, respectively.
They are based on convolution models and use, most of the time, the out-of-band intensities, i.e. the Infinium I probes in the color channel opposite their designed base extension, to measure the background.
For the kanamycin based extension by recombineering using pBALU-ext the procedure is shorter.
The bisulfite converted DNA was hybridized to the probes on the BeadChip followed by a single base extension to incorporate a fluorescent-labeled nucleotide (Cy3 or Cy5) at the target position to differentiate methylation status.
Window function implemented here should be understood as extensions to the physics based models.
Based on this model, two generalized extensions to the case of multiple VM migration were presented in [32,33].
