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To investigate if this novel mutation would influence recruitment of the telomerase complex, we employed a modified "supertelomerase" assay (ST) that used transient, plasmid based expression of the central scaffold protein of the telomerase ribonucleo-protein TERC and hemagglutinin (HA -tagged TERT in HA -tagged [ 32– 35].
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In this way, analytically based expressions of the solution of the considered class of BVP s can be derived by using classical quadrature rules, thus overcoming the need for cumbersome numerical techniques such as finite-difference or finite-element methods.
In this way, analytically based expressions of the solution of the considered class of BVP s can be derived by using classical quadrature rules, so overcoming the need for cumbersome numerical techniques such as finite-difference or finite-element methods.
The qRT-PCR based expression profiles of the selected genes were similar to that detected using Illumina-sequencing based method, and thus supports our results.
Functionally based expression profiling of the early mouse embryo is emerging as a powerful method to elucidate the regulatory networks that underlie developmental programs.
FPKM based expression values of the selected genes were also validated by qRT-PCR.
Eventually, reverse transcription Q-PCR based expression measurement of the 6-IA gene risk model genes was performed on a local cohort of 57 patients treated homogenously.
We present an efficient, marker-free, high-throughput, and multiplexed genome editing platform for industrial strains of S. cerevisiae that uses plasmid-based expression of the CRISPR/Cas9 endonuclease and multiple ribozyme-protected single guide RNAs.
11While the pseudomomentum flux itself ((overline {E^*-v^*v^*})) is diagnosable from model output, the pseudomomentum-flux-based expression of the energy flux ((overline {E^*-v^*v^*} omega ^*/k^*) is not E^*-v^*v^*} omegae from model output because of multiplication by the phase speed (see Appendix 3 for details).
Three other examples of the plant-based expression of the M2e epitope have been reported.
Plasmid-based expression of the ALAD−GFP fusion was driven by the HSP86 promoter.
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