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From these results, the original assembly file produced by Trinity was filtered to remove transcripts that represent less than 5% of the RSEM based expression level of its parent unigene or transcripts with transcripts per million (TPM) value below 0.5.
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The qRT-PCR based expression levels of all of these alternative splicing events displayed the same quantitative trend as earlier observed in the microarray analysis.
Adding weights based on expression level of a node leads to construction of response networks and hence identification of biologically significant paths [ 50].
Second, the significant anti-correlation between miRNA-mRNA pairs was determined based on the expression level of each aberrantly expressed miRNA and each aberrantly expressed mRNA.
Based on these findings, a clinical trial for anti-PD1 (pembrolizumab) was conducted where NSCLC patients were screened based on expression levels of PD-L1 on the primary tumor7,14.
A diagnostic model based on expression levels of ten miRNAs is constructed in the discovery set.
Based on expression levels of the top-ranked genes, we chose Col4a1/a2, encoding the alpha-1 or alpha-2 chain of type IV collagen, for the reporter gene in our shRNA screening (Figs 1A and S1B).
Alternatively, autosomal genes may regulate imprinting based on expression levels of heterosomal genes.
There was minimal mesoderm or neuroectoderm formation based on expression levels of the markers brachyury and Sox1, respectively.
Finally, we incorporated gene expression and biochemical pathway data to reduce the number of candidate gene products putatively participating in the eight steps of the photorespiration pathway from 52 to 10, based on expression levels of putatively functionally redundant genes.
Four different stromal subpopulations identified based on expression levels of Pdgfrα and GFP were isolated and analyzed by RNAseq.
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CEO of Professional Science Editing for Scientists @ prosciediting.com