Three additional mutational differences (two adjacent SNPs and a single base pair insertion) were found in FSL R2-574 during Sanger sequencing-based confirmation of the SNPs identified initially.
Quality control included Stability (shelf life), determination of pyrogenicity, sterility and cytotoxicity, confirmation of the absence of temperate bacteriophages and transmission electron microscopy-based confirmation of the presence of the expected virion morphologic particles as well as of their specific interaction with the target bacteria.
Unfortunately, the peritoneal fluid could not be sent for PCR-based confirmation of the species and antibiotic susceptibilities were not performed.
This study serves as the first sequence-based confirmation of the discord between nuclear and chloroplast phylogenies (see Soltis et al., 1991; Soltis and Kuzoff, 1995) and uncovers new discord beyond what has previously been observed.
Together, our sequence-based data and PCR-based confirmation of the absence of SPIN elements in the python (Feschotte C, unpublished data), in contrast to the abundance of recently inserted SPIN elements in the copperhead, provide compelling new evidence that, as with mammalian genomes (Pace et al. 2008; Gilbert et al. 2010), reptilian genomes have been differentially invaded by these elements.
This decision was based on the confirmation of the decay properties of daughter nuclei as well as the confirmatory experiments at RIKEN.
The diagnosis of pneumonia was based on radiologic confirmation of the condition.
Diagnosis with EPS is, by inference, based on the confirmation of the initial clinical suspicion through the demonstration of an inducible arrhythmia or conduction disturbance (Lu and Bergfeldt 2003).
The final inclusion of the 24 children was based upon a confirmation of the SM diagnosis after a home visit with a parental diagnostic interview and a child assessment to rule out severe intellectual problems.
Detection of anti-Th/To was based on IP confirmation of the 7-2 and 8-2 RNAs by RNA analysis with urea-PAGE and silver staining (Silver stain plus, Bio-Rad, Hercules, CA, USA).
Based on the confirmation of the presence of these antibodies, whole serum samples as well as enriched anti-SGH specific antibodies were used to screen our nymphal salivary gland library using phage display to identify proteins specifically recognized by these antibodies.
