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Briefly, single and multiple bead based assays were performed to determine the optimal concentration of the detection antibody, incubation times and reporter signal.
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To investigate the impact of hypoxia on cell proliferation and viability, four cell-based assays were performed on the three human breast cell lines exposed to either hypoxia or normoxia for 5 days.
Colony PCR-based assays were performed to detect whether salKR was still present in the genome or not.
Flow cytometer-based assays were performed for detecting apoptosis and cell cycle distribution.
All flow cytometry-based assays were performed on a BD FACS Canto II.
The fluorescence polarization (FP -based assays were perFP -based previously described.
To identify macrophage-specific secreted factors responsible for the increase in fibroblast proliferation, quantitative multiplex cytokine and chemokine ELISA-based assays were performed.
As a result, all subsequent plate-based assays were performed with 1 M stock solution of the chemical of interest onto the filter discs for the most pronounced observable result.
To further address the functional consequences of a possible link between ErbB2 and IGFBP3 regulation and a possible role in breast cancer, cell-based assays were performed in the ErbB2-overexpressing and invasive breast cancer cell line SKBR3 after knocking down ErbB2 and IGFBP3 expression.
To begin to characterize the FGFR2 transcripts that were present in SUM-52PE cells, PCR-based assays were performed to estimate the relative proportions of transcripts containing exon IIIb versus IIIc, and the proportion of C1/C2 variants as compared with C3 variants.
The development of new combinations was based on laboratorial tests and assays were performed independently and in a blind manner.
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