This emerging field of microbial or bacterial source tracking (MST or BST) often relies upon molecular biology-based assays to identify specific microorganisms and to link environmental microbiological pollution to its source.
However, it is extremely difficult to characterize this property in cellular models, primarily, because of the narrow time window between cyt. c release and caspase activation, and lack of sensitive cell-based assays to identify and distinguish direct caspase activation from indirect.
This test uses a two-channel ELISA-based assay to identify which of the two possible bases at the SNP position was added to a captured PCR amplicon in a single-base primer extension reaction.
However, development of assays to identify likely responders to a drug based on gene expression measurements has some inherent difficulties.
This seven-gene classifier could be used in a polymerase chain reaction-based clinical assay to identify ER- patients with a good prognosis, who may therefore benefit from less aggressive treatment regimens.
In this study, we developed a simple tuf gene based PCR and sequencing assay to identify isolates belonging to the genera Enterococcus, Staphylococcus, Streptococcus, and Lactococcus.
In this study, we developed a simple and economic, IFN-γ based high-throughput screening assay to identify natural products that could enhance the NK cells tumoricidal activity.
We used a microarray based micrococcal nuclease sensitivity assay to identify genome-wide changes in chromatin structure in mop1-1 immaturearsrs and observed an increase in chromatin accessibility at chromosome arms associated with loss of MOP1 function.
In vitro transport properties were studied with cell-based assays to identify the best substrate for OATP1B1.
Studies had to provide a clear description of the use of PCR-based assays to identify HPV DNA.
In this paper, we have employed bioactivity-based assays to identify anti-proliferative potential of the extracts of the medicinal plant P. urinaria using simple in vitro methods.
