Second, the cell viability was determined by MTT assays to evaluate the cellular toxicity of compounds.
We used several secondary genetic assays to evaluate candidate modifiers.
We also take advantage of dimer formation between the extracellular domain (ECD) of mGluR5 and design an ECD based surface-binding assay to evaluate dimerization and surface expression of mGluR5 containing various truncations or point mutations.
After extensive characterization, the BMP-g-nHA/PLGA hybrid nanofiber scaffolds were subjected to standard in vitro cell-based assays to evaluate their potential as new bone tissue regenerating and implanting materials.
However, despite their known limited metabolic capacity, in vitro cell lines still represent promising tools for the development of high-throughput, predictive and mechanism-based assays to evaluate the potential toxicity of agents such as NPs [ 26].
Such efforts will be very helpful in the development of immune-based assays to evaluate the clinical performance and utilities of aberrant glycoforms of TIMP-1 in a personalized approach to cancer diagnosis and treatment.
LAM has also been used as an antigen in development of antibody based assays and to evaluate antibody response kinetics on experimental inoculation but it is cross reactive to antibody elicited by non-tuberculous mycobacteria [ 39].
First, we used telo-FISH assay to evaluate telomere length.
We used traditional hemagglutinin inhibition assay to evaluate antibody titers.
Plasma aminotransferases were assayed to evaluate hepatotoxicity in Coilia nasusu.
