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Differential expression of CAMK2A has been observed in CSF samples in neurological disorders using mass spectrometry based assays such as multiple reaction monitoring (MRM) [ 52].
However, when less sensitive signal based assays such as HC2 were used, sensitivity of self-sampling was lower, and often also specificity was lower.
With future improvements, this device could be capable of performing light scatter based assays, such as relative white blood cell counts.
Syphilis testing is usually done using laboratory based assays such as Treponema pallidum haemagglutination assay (TPHA), Treponema pallidum particle agglutination assay (TPPA), rapid plasma reagin (RPR), or enzyme Immunoassay (EIA).
Methods to screen large chemical libraries for inhibitors of protein kinases include radiometric assays [ 3], ELISA [ 4], ATP consumption assays [ 5] and several fluorescence based assays such as time-resolved fluorescence (TRF) [ 6], fluorescence polarization (FP) [ 7, 8], fluorescence energy transfer (FRET) [ 9] or fluorescence quench assays [ 10].
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The choice of a blood based assay, such as mSEPT9, in a programme of CRC screening depends on multiple features not assessed in this study, including the potential for increased compliance, the necessary frequency of testing and features of competing tests, such as cost.
This review will describe the instrumentation used for noninvasive imaging of reporter genes, the reporter genes developed for noninvasive imaging with radio-nuclide-based assays such as positron emission tomography, and the reporter genes used for optically based noninvasive assays using sensitive charged-coupled device cameras.
DNA-mediated affinity-based assays, such as immuno-PCR and proximity ligation assays (PLA), use oligonucleotides attached to affinity reagents as reporter molecules.
Here, we report that classical dye-based assays such as MTT and neutral red (NR) that determine cell viability produce invalid results with some NM (nanomaterials) due to NM/dye interactions and/or NM adsorption of the dye/dye products.
Herein, the major viability-based assays, such as proliferation, necrosis, or apoptosis and DNA damage detection of GSH-GAuNps and LA-GAuNps in HBL-100 cells have been proved as keys to provide insights into their use in biomedicine.
This observation is important in the further development of aptamer-based assays, such as targeted therapy, apoptotic and viability assays at physiological conditions.
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