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A polymerase chain reaction-based assay is also used to differentiate the DENV serotypes.
Remarkably, a vast majority of molecules identified using this yeast-based assay were also active against mammalian prion ex vivo in various mammalian cell-based assays ([29] [31] and D. V. and M. B. unpublished results) and in vivo for the few that were tested (Tribouillard-Tanvier et al. in press).
One of these comigrated on gels with the major complex exhibiting cI activity, and cI activity based on this assay was also clearly decreased in Cox6a knockdown flies, though was hardly affected by the levy mutation.
The new SmD3 assay is also based on Sm peptide containing sDMA [ 14].
Since QFT-GIT assay is also based on IFN- γ response from TH1 T cells in response to MTB antigens stimulation, the sensitivity of the assay is compromised by helminth infection.
An alternative method based on the assessment of total protein using the Pierce Coomassie Plus (Bradford) Assay was also evaluated and compared.
The Renilla luciferase activity in the cell based reporter assays was also unaffected by up to 10 µM of EGCG (data not shown).
Qualitative Hg(0) volatilization assay was also performed as previously discussed (Oyetibo et al. 2015b) based on the method of Nakamura and Nakahara (1988).
The assay was also tested with a single nucleotide polymorphism target, changing one base at the ligation site.
MTT assay was also carried out.
This assay was also performed in triplicate.
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