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A mouse multiplex based assay was used to determine the indicated cytokine/chemokine plasma concentrations per the manufacturer's instructions (Bio-Rad, Hercules, CA) via Luminex 100 technology (Luminex, Austin, TX) essentially as previously described [3].
A PCR/digestion based assay was used for genotyping using the same primers as previously described [ 30] and subsequent restriction analysis using Hsp92II.
To study the effects of CDDO-Me on Stat3 nuclear translocation in live cells, a real-time cell based assay was used as described below.
A PCR based assay was used to determine the presence of the tnpA and tnpB genes, as well as of cagA, in 360 H. pylori strains isolated from H. pylori infected patients.
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In the cytotoxicity studies, interference caused by AuNPs with some typical cytotoxicity assays was demonstrated; thus, only data obtained from the resazurin based assay were used.
For example, in Ecuador, a simple polymerase chain reaction-based assay was used to diagnose leishmaniasis[ 58].
Next-generation sequencing based assays were used to generate genome wide SNP profiles, BRCA1/2 mutation screening, and BRCA1 promoter methylation data.
This could have potential implications given that NM, such as ultrafine superparamagnetic iron oxide nanoparticles (USPION), are increasingly finding their use in nanomedicine and the absorbance/fluorescence based assays are used to assess their toxicity.
Recently, a nanobody-based assay was used to demonstrate that RgpB could represent a novel diagnostic target, thereby simplifying.
This synaptic function-based assay was used to assess the efficacy of rationally selected drugs to restore neurotransmission in neurons comprehensively intoxicated by BoNT/A.
In contrast, when a luciferase-based assay was used to evaluate endothelial tube formation in the presence of MDC CM, AFDC CM, and non-conditioned Dulbecco's Modified Eagle's medium (DMEM), there was an increase in mean tube length in endothelial cells cultured in MDC CM compared to endothelial cells cultured in DMEM.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com