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A non-invasive miRNA based assay to detect bladder cancer in cell-free urine.
Using DyLight™-594 fluorescence-labeled rPGRN (DyL-rPGRN) and SORT1-expressing COS-1 (COS-1SORT1) cells, we established a cell-based assay to quantitatively analyze PGRN endocytosis by measuring DyLight™-594 emission from endocytosed DyL-rPGRN upon treatment.
TaqMan-based probe primer pairs were designed for each assay to quantitatively measure the messenger RNA (mRNA) expression levels of specific genes.
We have developed an assay using the branched DNA signal amplification assay (bDNA assay) to quantitatively measure the mRNA levels for human UCP1, 2, and 3. UCP-subtype-specific primers were designed for the assay.
In this study, we developed a sensitive and specific SYBR Green I real-time PCR assay to quantitatively detect ORFV.
Furthermore, we employed an apoptosis assay and a clonogenic survival assay to quantitatively compare the effects of the different therapeutic strategies.
We also used a FITC-Casein assay to quantitatively measure the activation of MMP-2.
We developed and validated the qISH assay to quantitatively assess the level of expression for miR34a using TMA and the IF-based AQUA technology.
Newman et al. present the first high resolution melt (HRM) assay to quantitatively analyze differences in murine DNA methylation levels utilizing CpG methylation of LINE1.
The visual-chip based assay should be ideal method to detection of aldulteration in CPM.
Purification of H2-Db class I MHC molecules by affinity chromatography, and performance of assays to quantitatively measure peptide affinity based on the inhibition of binding of a high affinity radiolabeled peptide to purified MHC molecules, has been detailed elsewhere.
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