We have systematically addressed epigenetic parameters of donor design, using a flow-based assay to quantify correction frequencies and expression levels of a green fluorescent protein (GFP) reporter gene in a human cell line.
We used the CellTiter 96 AQueous Kit® assay (Promega), an MTS-based assay, to quantify cell viability by measuring cellular metabolism.
We have developed a high-throughput scanning cytometry-based assay to quantify phagocytosis of S. aureus by adherent human blood-derived AM-like macrophages in a 96-well microplate format.
To extend the analysis, we used a flow cytometry-based assay to quantify infectious dengue particles released by MDA-MB-435-GFP and shDRBP76-GFP cells at 24, 36, and 48 hours after infection with dengue virus.
We use a cell culture-based assay to quantify miRNA abundance and determine that the sequences that direct this choice must be outside the miRNA duplex.
We measured the levels of phosphorylated eIF2α using an antiphospho-eIF2α antibody-based assay to quantify phosphorylation at serine 51 (see 'Materials and methods').
We used a combination of molecular dynamics (MD) simulations and a gramicidin-based assay to quantify the compounds' bilayer-modifying potency.
We used a Southern blot assay to quantify chimera composition based on the fact that cells derived from control (genotype TfamloxP/ TfamloxP) or MILON embryos (genotype TfamloxP/ TfamloxP, +/ CamKII- cre) are homozygous for the TfamloxP allele, whereas cells derived from LacZ embryos are homozygous for the wild-type (Tfam+) allele (Fig. 1B and C).
To resolve such potential difficulties of SYBR Green based analysis of loxP containing sequences we therefore developed an alternative assay to quantify 1lox/2lox allele ratios using a probe based Real-Time PCR approach.
The assay to quantify basal mitophagy in mammalian cells is based on differential stability of a tandem protein (mito-RFP-GFP) as previously described (Kim et al, 2013).
The assay to quantify IgG anti-pneumococcal antibody levels was based on the Procedures of the World Health Organization Pneumococcal Serology Reference Laboratories at the Institute of Child Health, University College, London, UK, and on the procedures of the Department of Pathology, University of Alabama at Birmingham, AL, USA [ 14].
