This test uses a two-channel ELISA-based assay to identify which of the two possible bases at the SNP position was added to a captured PCR amplicon in a single-base primer extension reaction.
We designed and set-up a MALDI-TOF MS-based assay to identify the most isolated and emerging therapy-refractory/uncommon fungi from cystic fibrosis (CF) and immunocompromised patients.
We developed a microchip-based assay to identify the most common AMD-associated ABCR gene variants (G1961E, D2177N) by use of the Nanogen Workstation; subsequently we report the results achieved using this chip in an ethnically homogeneous group of AMD patients.
We developed a quantitative plate-based assay to identify siRNA duplexes that modify cellular responses to the Tumor Necrosis Factor-α (TNF) cytokine.
As the first step, we selected ganR transfectants, which were then tested with a simple PCR-based assay to identify colonies (replacements) in which the reporter cassette had replaced the target cassette in the reversed orientation [9].
Significantly, this has allowed the development of a single-enzyme, colony-based assay to identify desired ω-TA activity.
Fifty-nine offspring were genotyped with a PCR-based assay to identify the BAC vector and flanking sequences.
Here, we report the application of a target-based assay to identify small molecules, which modulate the interaction between Gag and Ψ.
Our results also validate the use of the yeast-based assay to identify compounds potentially active in making EBV-infected cells targets for the immune system.
A PCR-based assay to identify SEPT9 methylation has been developed as an automated, commercially available kit, (Epi proColon Early Detection Assay, Epigenomics, Germany) which reportedly detects CRC with 70% sensitivity and 90% specificity [ 118].
If the sex of the cell/tissue donor is not known, it can be determined by a PCR-based assay to identify specific fragments of the X and Y chromosomes.
