The aim of the present commentary is to advocate the use of human DC based assays to evaluate vaccine immunogenicity for vaccine development and as alternative for animal testing for batch release.
To determine whether non-synonymous SNPs can alter RIG-I-induced antiviral and/or pro-inflammatory signaling pathways, we used a functional cell-based assay to evaluate RIG-I-dependent activation of an IFN-β promoter or an NF-κB or IRF-3-dependent promoter, respectively.
We next performed the reporter-based assay to evaluate the functional consequence of the predicted piRNA:mRNA interactions.
We used a simple urine-based assay to evaluate the prevalence of antihypertensive treatment non-adherence and its impact on blood pressure in a specialist hypertension centre.
32 Here, we used a novel and validated quantitative flow cytometry-based assay to evaluate changes in phosphoprotein expression in AML blasts.
To this end, we performed a cell-based assay to evaluate the inhibition of virus replication by three non-NAIs, T-705 (favipiravir), ribavirin and NT-300 (nitazoxanide), on the wild-type and the R292K mutant.
Here, we used a live cell-based assay to evaluate the epigenetic effects of the hypomethylating drug decitabine in combination with a number of anti-cancer drugs that are currently active in the clinic.
One way to approach this would be to work backward in other words, by knowing the identity of the specific neuronal elements one would utilize a metabolomics-based assay to evaluate whether bacteria in adjoin gut sections are capable of producing those neurochemicals that would interact with the neuronal elements.
We also take advantage of dimer formation between the extracellular domain (ECD) of mGluR5 and design an ECD based surface-binding assay to evaluate dimerization and surface expression of mGluR5 containing various truncations or point mutations.
LAM has also been used as an antigen in development of antibody based assays and to evaluate antibody response kinetics on experimental inoculation but it is cross reactive to antibody elicited by non-tuberculous mycobacteria [ 39].
First, we used telo-FISH assay to evaluate telomere length.
