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Therefore, it a PCR based assay to determine the presence/absence of a specific spacer sequence by designing primers that bind to conserved spacers that flanks a deletion site.
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Both trials are designed to assess the abilities of molecularly based assays to determine best adjuvant treatment for specific subsets of breast cancers, and in particular to determine which patients need chemotherapy and which are unlikely to benefit from chemotherapy.
Using a MTS-based assay to determine cell viability, we found that neither of these cytokines mediated resistance to PLX4032.
The chapter describes specifically, a fluorescence activated cell sorting (FACS -based assay to determine the eFACS -basedtivassayH-Ras and R-Ras on intogrin ligandetermine affinithe
In this study, we had developed an IFN-γ-based assay to determine NK cell killing activity in an in vitro NK cell-cancer cell interacting microenvironment.
We developed a PCR-based assay to determine if these genetic features were consistently present in a larger group of colonization or invasive isolates.
For example, one or more oligonucleotides specific for the same gene may be coupled to different microsphere sets, combined, and subjected to a hybridization-based assay to determine the best signal (specificity and sensitivity) for the desired set of analytes.
These hits were further screened by a high-content image-based assay to determine their ability to block in vitro P. yoelii liver-stage development (Meister et al., 2011).
It was employed to an ELISA-based assay to determine the protein activity of GAL4 domain for the TfRscFv-GAL4 fusion protein when bound and not bound to its special anti-GLA4 antibody.
We first analysed the N-terminal conformation change by a FACS-based assay to determine whether SB-218078 could block BAK N-terminal conformation change when cells were treated with the inhibitor followed by UV damage.
We next performed reporter-based assays to determine the requirement for specific piRNA:mRNA base-pairing interactions in MIWI-dependent regulation of mRNA stability.
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