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The main advantage of an SPR-based assay is that it is an extremely sensitive optical sensor, capable of detecting subnanogram levels in real time without any specific label [16].
A general limitation of the single most commonly used 4E6-based assay is that native LDL is also detected.
Another advantage of the ROS-based assay is that tree weakening resulting from pathogen exposure during testing could be curtailed by the elimination of the live pathogen.
The traditional interpretation of a result from a population-based assay is that it defines an expected, or mean, cellular behavior.
One major advantage of the FRET assay over the SDS-PAGE-based assay is that it can be easily configured to a high-throughput format for analysis or for screening.
One possible interpretation for the inconsistent results between the reconstituted β-secretase assay and the cell-based assay is that APP A673T is preferentially processed by an unknown enzyme, leading to a reduced level of sAPPβ from APP A673T cells.
Interestingly, the sensitivity of phage based assays was lower than that of microscopy in four studies [ 8, 9, 13, 15].
To learn more about how FXIIIa selects its targets, a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS -based assay was developed that could directly follow the consuMS -based assaytamine-containing substrate and the formation of a cross-linked product wash glycine ethylester.
We have overcome these challenges and developed a robust high throughput-compatible bioluminescence based assay that is amenable to siRNA based perturbation.
The IS6110_T assay provides a PCR based assay system that is compatible with current diagnostic protocols for the detection of M. bovis in the USA and compliments current testing strategies.
One drawback of the flow cytometry based assay described here is that it can only be performed in intact cells.
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