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Carbohydrate liposomal quartz crystal microbalance based assay has been established for studying carbohydratelectin binding.
A PCR based assay has been developed by the CDC to facilitate diagnosis.
EpCAM expression based assay has been FDA approved and widely used to detect circulating tumor cells in breast cancer [ 26].
A blood based assay has been described that demonstrates a failure to induce IFN-γ expression in renal transplant patients and differences in IL-10 and TNFα expression [ 56], which could be promising biomarkers to identify patients who could benefit from adjunctive immunotherapy.
To improve IHC assay sensitivity, a novel, non-endogenous hapten, 3-hydroxy-2-quinoxaline and tyramide amplification into a DAB-HRP based assay has been developed and detects low levels as well as heterogeneous ALK protein expression in NSCLC TMAs but it is not commercially available [ 27].
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Gene expression based assay have been extensively used by many laboratories for biodosimetry measurement of radio-responsive genes in human peripheral blood lymphocytes and is a potential method that fits the criteria to provide high-throughput data with an accurate measurement and in a timely manner [10 18].
Although concordance between this platform and qRT-PCR based assays has been reported in high quality specimens, information is lacking on comparisons in biofluids especially urine.
Minicircle based assays have been used as markers for T. cruzi diversity [ 33, 34].
Several molecular biology approaches, such as T7 in vitro transcription and PCR based assays, have been attempted [ 8- 10].
Various antibody based assays have been developed but these were tailored for animal cells and the early assays detected spurious components in non-animal cells [ 9].
IFN-γ based assays have been studied in the diagnosis of extrapulmonary TB [ 16, 19, 21], but few studies have included HIV positive patients [ 18, 20, 30].
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