A real-time PCR based assay for highly sensitive and accurate quantification of methylated SHOX2 copies in a background of unmethylated DNA was developed.
The procedure consisted of a sequence of cell disruption, starch precipitation using an aqueous solution of 80% ethanol, enzymatic hydrolysis of starch to glucose monomers using α-amylase and amyloglucosidase, and a spectrophotometric-based assay for quantification of glucose monomers.
In this study a new method was developed and validated for the absolute quantification of endogenous angiotensin II levels in human plasma, since recent studies demonstrated the insufficient specificity of the monoclonal antibodies using for the quantification of angiotensin II by commercial ELISA based assay [ 6, 7].
The SYBR Green methodology seems to be a better option for quantification than compared to sequence-specific fluorescent probes in the TaqMan or TaqMan MGB based assay, since it requires only one set of specific primers, hence providing additional experimental flexibility, and it reduces assay set-up and running costs while providing similar levels of accuracy in optimised assays [25].
Quantification of the LPS fraction was carried out using a colorimetric based assay [50].
In the present study, we describe an immuno-threshold-based assay for the quantification of cortisol.
One-step qRT-PCR was applied for the relative quantification of VEGF-A, VEGF-B, VEGF-C, VEGFR1, VEGFR2 and VEGFR3 mRNA expression, by using gene-specific TaqMan® based assays.
Herein we report a simple, highly reproducible, alternative approach for quantifying the kinetic stability of TTR tetramers in human plasma based on the previously reported subunit exchange assay for quantification of the kinetic stability of recombinant TTR in buffer.
Here we report a non-radioactive assay for quantification of cell-surface DNA binding based on the isoparametric analysis of flow cytometric data as described by Chatelier et al., Embo J., 5 (1986 11811986 1181
The present study investigated 120 naturally infected cultured shrimp samples by SYBR Green based qPCR assay for WSD diagnosis and quantification of WSSV load.
