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For detection of positive selection, codon-based analysis was implemented using codeml in PAML v3.15 [ 23].

A distance-based analysis was implemented by using the substitution model described above to estimate distances and the neighbour joining algorithm to infer a phylogeny, also using the PAUP* software.

Finally, a terrain classification scheme based on cluster analysis was implemented and the landscape of the study area was mapped on the basis of mountain geomorphometry.

Statistical analysis was implemented, based on unpaired Student's t-test criteria.

Base-pair level differential methylation analysis was implemented using the R package methylKit 0.9.2 [ 29].

Content analysis was implemented for further analysis based on their research topics, issues category, research settings and samplings, research designs, research method and data analysis.

Content analysis was implemented for further analysis based on their research topics, issues category, research settings and sampling, research design, research method and data analysis.

If blood gas analysis was implemented, pH ≤ 7.2, base excess ≤ -5 mmol/l and PaCO2 ≥ 50 mmHg defined a severe metabolic response.

The Bayesian analysis was implemented in MrBayes 2.3 (Ronquist and Huelsenbeck 2003) and was based on the model selected by MODELTEST above.

The stepwise regression analysis was implemented using STATISTICA 9.0 (Statsoft Inc).

The analysis was implemented using SAS Proc Mixed.

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