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Many published tools which identify new micro RNA genes use sequence and structure based alignments to find the best candidates [ 63].
Use pairwise structure based alignments to generate a template alignment of all PDB sequences.
We then aligned this structure-based alignment to the human UGT1A1 sequence based on the predicted vertebrate UGT secondary structure profile.
We then submitted this codon-based alignment to the SNAP server that employs the Nei and Gojobori method [ 85].
To this end, a randomly selected fraction of 10% of the reads that were not assigned to the prokaryotic genome database by nucleotide alignment were subjected to BLASTX-based alignment to the NCBI protein database.
G. barbadense SNPs developed in silico were randomly and nonrandomly (based on theoretical location on chromosomes 12 and 26 based on alignment to the D5 sequence) selected for primer design and experimental screening.
For cruzain, PDB entries 1aim, 1me4 and 1me3 were aligned with falcipain 2 using Modeller's structure based alignment algorithm to serve as templates for the complex.
Then this protein based alignment was converted to the codon-based alignment with PAL2NAL [ 45].
Cultivar-restricted SNPs are SNPs found exclusively in the two cultivars based on alignment to the reference genome.
The ancestral allele was based on alignment to the chimpanzee sequence.
Five scaffolds were reoriented in ChLGX based on alignment to the consensus genome maps (Fig. 8 a– b).
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