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The EDIII based ELISAs may be useful in epidemiological surveillance and vaccine efficacy trials.
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Direct stool microscopy, stool culture, duodenal biopsy, and serological tests (e.g. ELISA) may be useful.
For serum assays, ELISA may be more sensitive but Western blot is more specific.
Moreover, this suggests that different experimental measurement techniques, including qPCR and ELISA, may be applicable.
We argue that the ELISA may be used in animal field studies.
Thus ELISA may be used to confirm the diagnosis in acute and chronic phases [ 7- 9].
The observation that the results of EDIII-T based ELISAs were slightly better than those obtained with the mixture of EDIIIs argues against the possibility that the tetravalent design may have contributed to the lower sensitivity.
Some stakeholders requested guidance as to the availability of methods to determine markers of gluten in beverages such as beer, given that bioanalytical techniques based on ELISA may not be providing determining results whose purpose is, in particular, to clearly identify the source of gluten.
fMptD and MptD-peptide based ELISA were performed in 96-well microtitre plates (Maxisorp; Nunc-Immunoplate, Roskilde, Denmark).
The discrepancy between the dot blot and ELISA results may be due to differences in the analytical techniques.
Antibody titres and isotyping was done using HA protein based ELISAs.
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