Sentence examples for base was then added from inspiring English sources
10 mM Tris base was then added to the wells to solubilise the bound SRB dye, and the plates were then read at 492 nm using the Anthos 2001 plate read (Jencons Scientific Ltd ,Leighton Buzzard, UK).
A multiform or cork base was then added.
An adenine base was then added to the purified end repaired products using Klenow exo (3′ to 5′ exo minus) enzyme.
The plates were then air-dried.100 μL of 10 mM Tris base was then added to the wells to solubilise the dye.
An "A" base was then added to the 3' end of the blunt phosphorylated DNA fragments, which was used to ligate to the Illumina adapter with a single "T" base overhang at its 3' end.
This dNTP solution, now depleted of impurities of the dNTP complementary to the templating base, was then added to the mixture of polymerase and labeled DNA, as described above.
Pre-sheared salmon sperm DNA (8.8 μM in base pairs) was then added to the cuvette, incubated for 5 minutes, and again the fluorescence was determined; this constituted the maximal or 100% reading.
This estimate of the expenditure on the two selective COX-2 inhibitors (based on constant NAID volume) was then added to the actual expenditure on diclofenac, ibuprofen and naproxen (in the last quarter) in order to estimate what the total expenditure on these the five NSAIDs would have been (in the last quarter), if the total prescription volume for these five NSAIDs had remained constant.
The accumulated total of those unused seconds was then added to the base time to give the contestants their final question time limit.
The impact of the selective covariate on the key fixed effect parameters of the dose response model was then added to the base model to test the significance.
