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RNA template was then removed by RNase H, and double-stranded cDNA was prepared with DNA polymerase I. cDNA with blunt ends was created by T4 DNA polymerase and Klenow DNA polymerase and an 'A' base was subsequently added to the 3′ end of the blunt phosphorylated DNA fragments by Klenow fragment (3′ to 5′ exo minus).
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The extensive search strategy was also useful to detect ongoing RCTs, of which one [ 22] was subsequently added to the evidence base.
The PVP was subsequently added to the above solution.
Cement was subsequently added and the mixer was started for about 90 s.
Fresh medium was subsequently added, and the cells were shifted to 37 °C to allow synchronous infection.
This particular combination was subsequently added to the parametric coding amendment.
DAPI was subsequently added for nuclear staining.
HCl was subsequently added to adjust the pH to 7.4.
Hoechst 33258 was subsequently added for nuclear staining.
Enzyme conjugate was subsequently added for 30 min.
Thapsigargin was subsequently added to deplete intracellular stores.
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