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Every homopolymeric tract containing at least 8 guanines or cytosines in the NCTC11168 genome was variable with the exception of the 9 base tract in Cj1310c.
When guanine was present as a two base tract at the 5' and 3' ends of a decamer the result was the almost complete digestion of the adenine, thymine, and cytosine interstitial nucleotides, yet only a little over half of the guanine nucleotides were recovered.
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In Figure 1, we present the results of analyzing all 4 base tracts from Pf, Dm and Nc sequences as representative examples.
In this HT, deletion of one adenine in the 7-base tract was shown to result in inactivation of the gene.
We found no indel mutations for Cj1367c, containing a reportedly variable two base guanine tract, and Cj1677, with a 7 base thymine tract.
This CMBF protein contains so-called 'A.T hook' regions that interact with a 5 6 contiguous A T base pair tract.
The observed frequencies of non-overlapping base i tracts of length N,, in different DNA regions were analyzed as a function of tract length N in all 27 organisms.
Mutations were most commonly observed in a seven-base G tract around position g.chr1 26978524 (genomic coordinates refer to hg18) (c.5548), where there were six single base pair deletions and three duplications among gastric, colon, prostate, and pancreas carcinomas.
When there were three or four base guanine tracts at each end of a twenty base oligomer approximately 53% and 41% of the guanines were recovered, respectively.
We suspected that variations in the 8 13 base homopolymeric tracts of contingency loci would play a role in C. jejuni adaptation during serial passage.
In line with our results, Need et al. demonstrated no microstructural white matter changes in episodic and chronic migraine patients based on Tract-based spatial statistics (TBSS) analysis [22].
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