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Spatial expression patterns of the miRNAs and their target genes were found to be expressed in shoot tip and base tissues by qRT-PCR.
The correlations between the expression levels of these miRNAs and their target mRNAs in pear shoot tip and base tissues in response to high temperature were shown in Table 3.
It is likely that the differential regulation of these genes in crl1 versus WT results from the absence of CR meristems that may modify the auxin balance in the crl1 mutant stem base tissues.
To examine if high temperature-responsive miRNAs can regulate the expression of their target genes, the expression levels of selected miRNAs and their target mRNAs were analyzed from tip and base tissues of pear shoots.
The expression levels of viral genomic RNA, miRNAs and mRNAs of their predicted target genes in the shoot meristem tip and base tissues were analyzed to explore the possible roles of miRNA regulation in the high temperature-dependent decrease in virus titer.
In this study, we identified high temperature-altered pear miRNAs using the next generation sequencing technology, and futher molecularly characterized miRNA-mediated regulaton of target gene expression in the meristem tip and base tissues of in vitro-grown, ASGV-infected pear shoots under different temperatures.
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Analysis of tongue base tissue revealed Trichinella larvae in 122, indicating a prevalence of infection of 16.5%.
We also analyzed expression patterns of several miRNA-mediated target genes in shoot base tissue.
This shows that the changes in mRNA levels in stem base tissue and in shoot tip tissue are different.
In addition, the other target genes showed the opposite expression patterns in base tissue compared with tip tissues (Fig. 8 and Table 3).
Gli-1 and Gli-3 gene expression was measured in microdissected base tissue from gastric units of InsGas mice with/without H. felis infection.
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