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Limited sequencing analysis of mutant clones from 0.25 or 1 Gy exposures revealed a higher frequency of deletions and multiple base sequencing changes with increasing dose.
Base sequencing error rates λ = 0.05 and 0.01 were considered.
Single-end, 100 base sequencing generated between 7 and 12 million reads per library.
Read depth was determined by calculating median base sequencing depth from the alignreads output.
The final concentration was verified by Quant-iT dsDNA HS Assay prior to Illumina HiSeq2000 PE 75 base sequencing.
Paired end 50 base sequencing by synthesis was performed using TruSeq SBS kit v3 (Illumina) on an Illumina HiSeq 1000 using protocols defined by the manufacturer.
Similar(48)
The relationship between the base sequence and the amino-acid sequence constitutes the genetic code.
In some organisms, methylation helps to eliminate incorrect base sequences introduced during DNA replication.
Different genotypes - the number of individuals with different base sequences.
This is obtained by cyclically shifting the base sequence.
After developing a way to include executable code in the base sequence, they set about making the exploit itself.
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