The exception was found in the 10 base resolution analysis for C. jejuni, where GenomiPhi and Repli-G exchanged position; while significant, the difference between the Repli-G and GenomiPhi samples in this instance was slight (0.005).
Deep sequencing technologies now enable whole genome single base resolution analysis of DNA methylation (Cokus et al., 2008; Lister et al., 2008), thus enabling global assessment of changes in DNA methylation in response to environmental and developmental cues.
Neri, F. et al. Single-base resolution analysis of 5-formyl and 5-carboxyl cytosine reveals promoter DNA methylation dynamics.
This descriptive study demonstrates that the RNA-Seq technique, if supported by adequate bioinformatic resources, provides promising new opportunities for a cost-efficient, single-base resolution analysis of the transcriptome complexity of leukemia cells, from both mutational and gene-expression perspectives.
Single-base resolution analysis of active DNA demethylation using methylase-assisted bisulfite sequencing.
3. Wu, H., et al. Single-base resolution analysis of active DNA demethylation using methylase-assisted bisulfite sequencing.
To ensure that CoBRA digests were representative of high methylation status in tumours, we carried out base-resolution analysis of promoter region methylation for BNC1, CCDC8 and GALNT9 by cloning and sequencing individual bisulfite-modified alleles from select tumours (Additional file 9: Figure S5).
Yu, M. et al. Base-resolution analysis of 5-hydroxymethylcytosine in the mammalian genome.
Xia, B. et al. Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale.
It will be also useful for base-resolution analysis of 5-hydroxymethylcytosine when applied to genomic DNA treated with adequate oxidants or enzymes.
