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Porschke, D. & Eigen, M. Co-operative non-enzymic base recognition.
The cleft contains depressions, suggesting base recognition occurs in a knob-into-hole fashion.
The binding mode of RBD1-G53V is altered, having lost its salt-dependence and displaying site-independence of base recognition.
We found that the binding of ssDNA homopolymers conformed to the specific code for base recognition established previously for RNA (Fig. 1e).
Besides mediating sequence-specific base recognition, the amino acid substitutions introduced in the SpCas9 variants facilitate conformational remodeling of the PAM region of the bound DNA.
Therefore, the code for base recognition by PPRs is maintained when binding ssDNA targets, opening the way for the use of cPPRs to target ssDNAs in a programmable manner.
Similar(33)
The conserved modes of cPPR-base recognition between RNA and ssDNA are reflected in the cPPR's ability to bind both ssDNA and RNA (Fig. 1c, e).
A 31-base long molecular beacon, containing a 15-base recognition sequence specific for the mutant target, was linked via a thiol modified poly thymine linker (10 bases long) to a 13 nm gold nano-particle and was exposed to mutant and wild type targets, and a clear differentiation was achieved at target concentrations as low as 1 nM.
At LTM, this pattern was accompanied by impaired familiarity based recognition.
The results are compared with a holistic Gabor filter bank based recognition methods.
The atlas based recognition method was evaluated using three manually pre-segmented 3D CT data sets of the hip.
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