Sentence examples for base quality values were from inspiring English sources

Polybayes likely suffered from the relatively large number of ESTs obtained in GeneBank for which no base quality values were available.

Base quality values are used in computation of overlaps and construction of multiple sequence alignments.

This is because the error rate was so high, and the base quality values are so poor that the mapping quality scores were always extremely low.

Nucleotide sequences and per-base quality values were extracted from the ABI-generated chromatograph files (SCF and AB1 files) using the phred base-calling program and evaluated for 3 features: 1) overall sequence quality (phred q-score >25); 2) percent of sequence (in nt) over q20 > 50%, and 3) the quality-trimmed EST insert length of more than 100 bp [ 79].

Nucleotide sequences and per-base quality values were extracted from the ABI-generated chromatograph files (SCF files) using the Phred-base-calling program and evaluated for 3 features: 1) overall sequence quality (Phred q-score >25); 2) percent of sequence (in nt) over q20 > 50%, and 3) the quality-trimmed EST insert length of more than 100 bp [ 37, 38].

While these base-quality values are not as accurate as Phred scores for Sanger sequencing, they do contain useful information about the accuracy of individual base calls.

Additionally, 31 bases with low quality values were trimmed from the 3′ end of each of the sequences, following parameters described earlier [ 22].

Bases sequenced and corresponding quality values were called and delivered in standard format by GS FLX for downstream bioinformatic analyses.

Base calling and attribution of quality values were performed using Phred [ 40], using default parameters.

Bases sequenced and the corresponding quality values were called and delivered in a standard format by GS-FLX-Titanium system for downstream bioinformatic analyses.

Quality values were assigned based on correlation cut-offs.