Sentence examples for base quality values of from inspiring English sources

This last feature is accomplished by mutating the simulated reads from the target genome using the base quality values of the selected Conus reads.

Base quality values of EST sequences without available sequencer raw data was set arbitrarily to a low value, 10%, and in the case of seed sequences to a higher confidence value of 50%.

Sequences derived from the genome project were assigned a base quality value of 40 (1 error per 10,000 bases), based on the fact that they are derived from an ~11X coverage of the genome.

These arbitrary quality values were derived based on the estimated sequencing error rate for the different types of sequences used: EST sequences were assigned an arbitrary base quality value of 20 (1 error per 100 bases), which is typical for single-pass, unedited sequences; while sequences derived from individual submissions to GenBank were assigned a quality value of 30 (1 error per 1000 bases).

As shown in Figure 1a and Figure 2a, although both the E. coli and B. cereus datasets have bases quality values of 80% or above at Q30 (Table 1), the E. coli dataset has a peak at minimal quality value 9 with 51%, and the B. cereus dataset at minimal quality value 8 with 26% in terms of reads' minimal quality statistics.

PolyBayes can use individual base quality values for the calculation of SNP probabilities, but because original chromatogram data is not available for all sequences, we have assigned arbitrary base quality values to these sequences.

ART uses empirical base quality profiles to accurately mimic the characteristics of Illumina hardware, and we use these base quality values to calibrate the machine learning framework.

Reads were quality-filtered by discarding adapter pollutions and the reads containing >50% low-quality bases (quality value of ≤5) were removing.

Base quality values are used in computation of overlaps and construction of multiple sequence alignments.

Firstly, the Raw SFF file was submitted to SFFTrim (Torrent Suite 3.2.1) and reads were trimmed when the average base quality values in a window size of 10 were less than 25 (Q-value of 25).

PHRED software [ 65] was used for base calling and assignment of base quality values, and vector and low quality-sequences were removed from the reads on the Egene platform [ 66].