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Our cut off for base quality control was Phred quality score of 20.
Local read realignment around indels, base quality score recalibration, and variant calling with GATK v2.8.1.
Furthermore, we performed base quality score recalibration with GATK to avoid system bias.
SNPs with base coverage of <10×, base quality of <25 or heterozygosity of >0.2 were removed.
Local realignment and base quality score recalibration were done using GATK.
(a) Base quality using Phred scores for the data from Illumina Miseq sequencing.
Knowledge Base Quality Analysis; RKF Principal Investigators Meeting; McLean, VA; October 19 , 2001
In addition, various approaches concerning case base quality are demonstrated.
Individual sequence reads with the base quality scores were produced by Solexa.
Evidence base quality was assessed using GRADE.
We used a minimum base quality allowed of 25.
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