Then newness, creativity and quality are generated.
A total of 66,702 and 74,018 DNA sequence reads with more than 100 contiguous high-quality bases were generated from samples Air-1 and Air-2, respectively.
Bases and quality scores were generated with the Ibis base caller [ 73].
Quality scores for the bases were generated by using the GATK's base quality score recalibration pipeline[ 10], starting with a default original base quality of Q20 for every base in the read.
Base calling and quality scores were generated with PHRED.
More than 100 million high quality reads of 101 base pairs (bp) were generated from PGT, leaf-PGT and leaf (Additional file 2).
High-quality partial CO1 gene sequences of 996 1,027 base pairs (bp) were generated for the seven successfully amplified specimens and were submitted to GenBank (accession numbers KM248530-248536).
Sequencing adaptors were trimmed using the software, and then high-quality (> 99.5% accuracy on single base reads) reads were generated (using default parameters).
After base-calling, trimming the vector sequence and eliminating low quality sequences, 978 and 1,069 high quality 5' ESTs were generated (Table 1).
After trimming adapter sequences and removing short sequences of less than 50 bases, a total of 910,607 high-quality reads were generated from the four cDNA libraries.
