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The 13,960 (for each of the conditions A to D) and 33,504 (for condition E) base pairs were sequenced.
From single colonies of the isolate, two replicates of the 16S rRNA gene (approximately 1,500 base pairs) were sequenced using Sanger's method.
433 base pairs were sequenced from 106 individuals among 10 owl species.
A total of 52,445 base pairs were sequenced, and 579 polymorphisms (out of which 207 were singletons) were observed (Table 1).
Nearly 2000 base pairs were sequenced on both DNA strands and a single base pair substitution was observed between sctt embryos and the wild type controls: an adenine within intron 4 was mutated to a thymine (Figure 2A).
Genetic data, totaling 6172 base pairs, were sequenced from 422 taxa.
Similar(53)
Usually, longer fragments (several hundred base pairs) are sequenced from both ends (paired-end sequencing) to provide additional information on correct read placement in the assembly.
The cloned structural gene (5130 base pairs) was sequenced and compared with the SIGLEC 1 in the National Center for Biotechnology Information (NCBI) database (accession number NM_023068).
For 1, N-PdG, the conformational equilibrium at the 3′-neighbor base pair is sequence-dependent.
PCR products larger than 200 base pairs were collected and sequenced using Applied Biosystems 3730XL Sanger sequencing with BigDye terminator serviced by Beckman Coulter genomics (Essex, United Kingdom).
In total, 1.24% of the 7,451,304 sequenced base pairs were repetitive elements.
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