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Primers were designed using Primer 3 software [ 20] to cover at least 20 base pairs at each 5' and 3' side of the exons.
Many of the amplicons within a cluster will vary by just a few base pairs at each end, and therefore do not represent truly different assays.
When these common SNPs were found, the quality of their base pairs at each point was evaluated, and the respective sequences were removed and rearranged in a new cluster.
For chemically synthesized siRNA, it was first found that the high free energy of first 5 base pairs at each terminal correlated well with siRNA efficacy [ 12, 25], and later other researchers discovered that it might be the high free energy of the first 2 base pairs [ 19, 20].
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We determined Nbp, the number of IRs that overlap the base pair at each location in each sequence.
The other base pairs, except for one base pair at each end, retain normal Watson Crick hydrogen bonding, as supported by NOEs between imino and amino protons.
Strains TA102 and TA104 contain AT base pairs at the hisG428 mutant site.
For this purpose, enrichment was defined as the ratio of IP reads to input reads, with reads summed over the 50 base pairs at the center of each binding peak.
This bend results almost entirely from two 40 degrees kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex.
Applied Biosystems approach works by chopping the genome up into 200 million pieces that are each about 1,500 DNA base pairs long, and then sequencing just the 25 base pairs at the edges of these pieces, yielding pairs of mated tags.
A simple biochemical procedure for producing small deletions (15 to 50 base pairs) at virtually any location in simian virus 40 DNA has been developed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com