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Under these conditions, it might be more favorable for a (partially) melted duplex region to base pair with a complementary strand in another synapsed quadruplex.
For UUR codons, because the anticodon wobble site is U and form Watson-Crick base pair with A, we also expect UUA codon to be preferred against UUG codons.
As a simple model of DNA evolution, the Jukes-Cantor formula [33] is applied to estimate a probability of change for each base pair, with a customizable transition rate α (0.001 by default) and time t based on the edge weights.
Average SNP density was 0.0185 per base pair with a standard deviation of 0.0238.
When the trimmer encountered a base pair with a quality less than 20, the closest read end was trimmed up to that base.
It has also been shown that replacing the G31 C39 base pair with a T31 T39 pair in the yeast initiatior tRNA renders it being able to participate in the elongation phase.
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All DNA polymerases can accommodate a Hoogsteen base pair with an 8-oxoG syn -A anti) mispair much more effectively than with a G-A mispair.
They found that the AF adduct exhibited a syn conformation at the preinsertion site (n – 1) and underwent a transition to an anti-conformation at the insertion site (n), allowing it to base pair with an incoming dCTP.
Implementation of the cSBL approach could potentially increase reads to twenty-three base pairs, or forty-six total base pairs with a mate-paired constructed library.
Primers designed to amplify the CA repeat detect 16 alleles from 121 to 151 base pairs, with a heterozygosity of 0.74.
For instance, in RNA interference, a short interfering RNA molecule (siRNA) forms complementary base pairs with a target region in mRNA, allowing a protein complex called RISC (RNA-induced silencing complex) to attach and cleave the target region [9, 10].
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