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As predicted, the total base pair length of rare deletions negatively and significantly (p = .01) covaried with FSIQ.
The concentration (pg/µl) was then used to calculate the number of molecules/µl of the final product: [single strand DNA (pg/µl)]/[MW of nucleotide (325) x base pair length of DNA strand] x [6.02×1023].
The frequency of mutations reported in this manuscript was calculated for each sample with this formula: Total number of detected mutations/(base pair length of the amplified fragments × number of sequenced clones) × 1000, considering that all analyzed samples were produced with the same number of PCR amplification cycles.
The base pair length of each allele was determined by running all samples with three marker individuals and a Genescan™-350 TAMRA size standard (Applied Biosystems).
Marker individuals, the TAMRA ladder and the slippage from incomplete PCR amplification were utilized to compute the base pair length of all other alleles.
We next combined HSVs co-locating within a region of gene sequence less than the 51 base pair length of an individual sequence read to produce haplotypes.
Similar(51)
Crosslinking between nucleic acid and proteins might be another reason, resulting in base pair lengths of approximately 200 bp or less, as this has been described in samples treated with formaldehyde [ 23].
Our results were in good agreement with the base pair length dependence of the on-time as previously reported (Fig. 2b 15.
Steve Turner, the company's founder and chief technology officer, wowed the audience with a video of single molecule sequencing in progress, the product of a proof-of-principle experiment reading a 150-base-pair length of DNA.
A biotinylated, double-stranded oligonucleotide of approximately 30 base pair length containing a TF-binding motif of interest is immobilized on streptavidin beads.
The slides chosen for this study were 22,000 element Compugen array chips which were spotted with single stranded oligonucleotides of 60 base pair length.
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