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Likewise, we call di,jNA(S2) the set of base pair distances between each of the molecules in populations NA and S2 for each of the realizations.
We focus our analyses in the three populations E, NA, and A. In the following, we will call di,jE(S1) the set of base pair distances between each molecule i in populations E and the target structure S1 for each realization j.
Mean, median and standard deviation for base pair distances between neighboring SNPs across chromosomes are in Table S2 (See Additional file 2: Table S2).
Conversely, δ does not depend on the base pair distances between the dominating structure in the two ensembles but only on whether the dominating structures are identical or distinct.
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A Perl script was used to compute the genomic base pair distance between each binding event and the closest transcription start site.
Analogously, we define di,jA(S2) as the base pair distance between molecules in the adapting populations A and the target structure S2 for each realization j.
A simple and practical candidate for such a measure is the base pair distance between the starting and stopping structures.
The constant l is a scale factor, since the maximum base pair distance between two molecules of length l is proportional to l.
The 9 base pair distance between these key adenosine residues was also investigated, and we found either increasing this to 12 nucleotides or decreasing it to 6 base pairs diminished GlnR binding.
The base pair distance between two secondary structures is given by the number of base pairs that have to be opened and closed to transform one structure into the other (as implemented in the RNAfold algorithm [ 18]).
When only two genome anchoring markers were available for a LG, the ratio R was calculated as the base pair distance between the two markers divided by the genetic distance between these two markers.
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