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We found that the identity of the central base pair (bp) of κB sites profoundly affects the transcriptional activity of NF-κB dimers.
The resulting heterologous polymerases EσA and EσB were then subjected to single round run-off transcription in the presence of standardized amount of heparin (in order to stop transcription after one round), on a linear 967 bp DNA fragment containing 767 bp upstream msdps2 promoter region and 200 base pair (bp) of the gene.
Using primers PgpdA-F1 and PgpdA-R1, 1314 base pair (bp) of gpdA promoter was amplified from plasmid pAN7-1.
All PCR primer pairs were designed for an mRNA sequence within 300 base pair (bp) of the poly(A) signal of each Indicator gene.
Reverse transcription-polymerase chain reaction (RT-PCR) for 300 base pair (bp) of the β-actin gene was used for quality control of these samples.
A typical TALE DNA-binding domain consists of a series of 33- to 35-amino acid repeats that each recognizes a single base pair (bp) of DNA via two adjacent amino acid residues, termed repeat variable diresidues (RVDs).
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The aligned matrix of the 43 taxa of the ITS region includes a total of 691 positions, 190 base pairs (bp) of which are phylogenetically informative.
Phylogenetic relationships were inferred from analysis of 1990 base pairs (bp) of mitochondrial DNA (mtDNA), represented by the complete nucleotide sequences of the cytochrome b (cyt b) and the ATP synthase 8 and 6 (ATPase 8/6) genes.
We designed a primer set to amplify part of the mitochondrial cyt b gene of both species and obtained 1024 base pairs (bp) of polymerase chain reaction (PCR) product.
The structure of nucleosome the basic unit of chromatin has been defined by crystal X-ray studies at high resolution; it consists of 147 base pairs (bp) of DNA wrapping around an octamer of histones (two copies each of H2A, H2B, H3 and H4) approximately 1.7 times (Luger et al., 1997).
Management-oriented genetic studies use primarily (1) genotypes from microsatellite loci within the 3×109 or so base pairs (bp) of the mammalian nuclear genome or (2) DNA sequence data from a portion of the 1.6×104 bp of the mitochondrial genome; the subsequence is also known as a haplotype.
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